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Addgene

pCMV-SUN2 GFP
(Plasmid #187684)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187684 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV-GFP
  • Backbone manufacturer
    Connie Cepko Addgene #11153
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SUN2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2193
  • Mutation
    WT
  • Entrez Gene
    Sun2 (a.k.a. B230369L08Rik, C030011B15, Unc84b)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (unknown if destroyed)
  • 3′ cloning site AgeI (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Lifeact sequence removed from vector backbone and replaced with gene insert

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-SUN2 GFP was a gift from Mary Beckerle (Addgene plasmid # 187684 ; http://n2t.net/addgene:187684 ; RRID:Addgene_187684)
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