pCMV-Pom121 GFP
(Plasmid
#187680)
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PurposeIn vivo visualization of NPC protein Pom121
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 187680 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV-GFP
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Backbone manufacturerConnie Cepko Addgene #11153
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePom121
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SpeciesM. musculus (mouse)
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Insert Size (bp)3600
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MutationWT
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Entrez GenePom121 (a.k.a. 2610027A18Rik, C80273, mKIAA0618)
- Promoter CMV
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Tag
/ Fusion Protein
- EGFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (unknown if destroyed)
- 3′ cloning site AgeI (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-Pom121 GFP was a gift from Mary Beckerle (Addgene plasmid # 187680 ; http://n2t.net/addgene:187680 ; RRID:Addgene_187680)