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Addgene

pU6-gRNA(SFTPCI73Tcorr)-SpCas9(BB)-2A-GFP
(Plasmid #187647)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187647 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSpCas9(BB)-2A-GFP backbone
  • Backbone manufacturer
    Addgene
  • Total vector size (bp) 9292
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    I73T gRNA
  • gRNA/shRNA sequence
    5’ CAG GTT CTG GAG ATG AGC AC 3’
  • Species
    H. sapiens (human)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pU6-gRNA(SFTPCI73Tcorr)-SpCas9(BB)-2A-GFP was a gift from Darrell Kotton (Addgene plasmid # 187647 ; http://n2t.net/addgene:187647 ; RRID:Addgene_187647)

  • For your References section:

    Patient-specific iPSCs carrying an SFTPC mutation reveal the intrinsic alveolar epithelial dysfunction at the inception of interstitial lung disease. Alysandratos KD, Russo SJ, Petcherski A, Taddeo EP, Acin-Perez R, Villacorta-Martin C, Jean JC, Mulugeta S, Rodriguez LR, Blum BC, Hekman RM, Hix OT, Minakin K, Vedaie M, Kook S, Tilston-Lunel AM, Varelas X, Wambach JA, Cole FS, Hamvas A, Young LR, Liesa M, Emili A, Guttentag SH, Shirihai OS, Beers MF, Kotton DN. Cell Rep. 2021 Aug 31;36(9):109636. doi: 10.1016/j.celrep.2021.109636. 10.1016/j.celrep.2021.109636 PubMed 34469722
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