LV-tetO-EF1a-GFP
(Plasmid
#187645)
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PurposeLentiviral vector expressing GFP from EF1a promoter downstream from tetO sites
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 187645 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneFUdeltaGW
- Total vector size (bp) 9107
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Modifications to backbonepLV-tetO-Sox2 (Plasmid #19765) digested with KpnI. Ef1a-GFP-WPRE PCR from LRG (Lenti_sgRNA_EFS_GFP) (Plasmid #65656). Gibson clone
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGFP
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Insert Size (bp)1610
- Promoter EF1a
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Tag
/ Fusion Protein
- GFP
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer TCGAGTGGCTCCGGTGCCCGTCAGTGGGC (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LV-tetO-EF1a-GFP was a gift from Martin Carroll (Addgene plasmid # 187645 ; http://n2t.net/addgene:187645 ; RRID:Addgene_187645)
