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pLenti-EF1a-CDK6-mutPAM-PGK-puro
(Plasmid #187638)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187638 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti-EF1α-gate-3HA-PGK-Puromycin
  • Backbone manufacturer
    Gift from G. Lu
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CDK6
  • Alt name
    Cyclin Dependent Kinase 6
  • Species
    H. sapiens (human)
  • Mutation
    PAM site mutation
  • Entrez Gene
    CDK6 (a.k.a. MCPH12, PLSTIRE)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    N. Persky, Broad Institute

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-EF1a-CDK6-mutPAM-PGK-puro was a gift from William Kaelin (Addgene plasmid # 187638 ; http://n2t.net/addgene:187638 ; RRID:Addgene_187638)

  • For your References section:

    HIF-independent synthetic lethality between CDK4/6 inhibition and VHL loss across species. Nicholson HE, Tariq Z, Housden BE, Jennings RB, Stransky LA, Perrimon N, Signoretti S, Kaelin WG Jr. Sci Signal. 2019 Oct 1;12(601). pii: 12/601/eaay0482. doi: 10.1126/scisignal.aay0482. 10.1126/scisignal.aay0482 PubMed 31575731
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