pDae079 (eMutaT7[transition])
(Plasmid #187622)

• Purpose: Expresses eMutaT7[TadA-8e], eMutaT7[PmCDA1] and UGI for mutagenizing genes downstream from a T7 promoter in e.coli
• Depositing Lab: Seokhee Kim
• Publication: Seo et al Nucleic Acids Res. 2023 Apr 18:gkad266. doi: 10.1093/nar/gkad266.

Backbone

• Vector backbone: pBAD33
• Backbone size w/o insert (bp): 5814
• Total vector size (bp): 12653
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: TadA-8e-T7RNAP
• Alt name: eMutaT7[TadA-8e]
• Species: Synthetic
• Insert Size (bp): 3198

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: 5'-ggatcattttgcgcttcagcc-3'
• 3′ sequencing primer: 5'-gagtatgaaaagtctgccgcc-3'

Gene/Insert 2

• Gene/Insert name: PmCDA1-T7RNAP
• Alt name: eMutaT7[PmCDA1]
• Species: Synthetic
• Insert Size (bp): 3641

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: 5'-cctggcggcagacttttcatac-3'
• 3′ sequencing primer: 5'-cagcatcttttactttcaccagc-3'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2022.06.09.495438v2 for bioRxiv preprint.