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Addgene

pDae029 (eMutaT7[TadA-8e])
(Plasmid #187620)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187620 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAD33
  • Backbone size w/o insert (bp) 5343
  • Total vector size (bp) 8541
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    TadA-8e-T7RNAP
  • Alt name
    eMutaT7[TadA-8e]
  • Species
    Synthetic
  • Insert Size (bp)
    3198

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer pBAD-F (5'-ATGCCATAGCATTTTTATCC-3')
  • 3′ sequencing primer pBAD-R (5'-GATTTAATCTGTATCAGG-3')
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDae029 (eMutaT7[TadA-8e]) was a gift from Seokhee Kim (Addgene plasmid # 187620 ; http://n2t.net/addgene:187620 ; RRID:Addgene_187620)

  • For your References section:

    A dual gene-specific mutator system installs all transition mutations at similar frequencies in vivo. Seo D, Koh B, Eom GE, Kim HW, Kim S. Nucleic Acids Res. 2023 Apr 18:gkad266. doi: 10.1093/nar/gkad266. 10.1093/nar/gkad266 PubMed 37070179
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