MSCV eIF4E W73A IRES GFP
(Plasmid
#18762)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 18762 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneMSCV-IRES-GFP
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Vector typeMammalian Expression, Retroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameeIF4E W73A
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Alt nameeIF4E
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SpeciesM. musculus (mouse)
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Insert Size (bp)1327
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MutationW73A
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Entrez GeneEif4e (a.k.a. EG668879, Eif4e-ps, If4e, eIF-4E)
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Tag
/ Fusion Protein
- IRES GFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer pLXSN 5' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MSCV eIF4E W73A IRES GFP was a gift from Scott Lowe (Addgene plasmid # 18762 ; http://n2t.net/addgene:18762 ; RRID:Addgene_18762) -
For your References section:
Dissecting eIF4E action in tumorigenesis. Wendel HG, Silva RL, Malina A, Mills JR, Zhu H, Ueda T, Watanabe-Fukunaga R, Fukunaga R, Teruya-Feldstein J, Pelletier J, Lowe SW. Genes Dev. 2007 Dec 15. 21(24):3232-7. 10.1101/gad.1604407 PubMed 18055695
Map uploaded by the depositor.
