pLV-EF1A-WWE(R163A)-HA-nLuc-FLAG-T2A-WWE-myc-cLuc-FLAG-IRES-Puro
(Plasmid #187612)

• Purpose: Split luciferase construct with nLuc fused to C-terminus of WWE domain with Arg163Ala mutation, linked by T2A to cLuc fused to C-terminus of WWE domain, linked by IRES to puromycin resistance cassette
• Depositing Lab: Robert Sobol
• Publication: Koczor et al Cancers (Basel). 2022 Jul 28;14(15). pii: cancers14153676. doi: 10.3390/cancers14153676.

Backbone

• Vector backbone: Lentivirus
• Backbone manufacturer: VectorBuilder
• Total vector size (bp): 11052
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: WWE domain (R163A) with C-terminal nLuc
• Species: H. sapiens (human)
• Insert Size (bp): 1608
• Mutation: Arg163Ala
• Promoter: EF1A
• Tags / Fusion Proteins:
  • HA (C terminal on insert)
  • Split luciferase (nLuc) (C terminal on insert)
  • FLAG (C terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Gene/Insert 2

• Gene/Insert name: WWE domain with C-terminal cLuc
• Species: H. sapiens (human)
• Insert Size (bp): 822
• Promoter: EF1A
• Tags / Fusion Proteins:
  • myc (C terminal on insert)
  • Split luciferase (cLuc) (C terminal on insert)
  • FLAG (C terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Resource Information

• Supplemental Documents:
  • pLV-EF1A-WWE(R163A)-HA-nLuc-FLAG-T2A-WWE-myc-cLuc-FLAG-IRES-Puro.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry