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Addgene

MSCV eIF4E IRES GFP
(Plasmid #18761)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 18761 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MSCV-IRES-GFP
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    eIF4E
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    700
  • Entrez Gene
    Eif4e (a.k.a. EG668879, Eif4e-ps, If4e, eIF-4E)
  • Tag / Fusion Protein
    • IRES GFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer pLXSN 5'
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MSCV eIF4E IRES GFP was a gift from Scott Lowe (Addgene plasmid # 18761 ; http://n2t.net/addgene:18761 ; RRID:Addgene_18761)

  • For your References section:

    Survival signalling by Akt and eIF4E in oncogenesis and cancer therapy. Wendel HG, De Stanchina E, Fridman JS, Malina A, Ray S, Kogan S, Cordon-Cardo C, Pelletier J, Lowe SW. Nature. 2004 Mar 18. 428(6980):332-7. 10.1038/nature02369 PubMed 15029198
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