pLV-EF1A-LivePAR(R163A)-Hygro
(Plasmid #187609)

• Purpose: EGFP fused to the C-terminus of a WWE domain containing the mutation Arg163Ala & a hygromycin resistance cassette.
• Depositing Lab: Robert Sobol
• Publication: Koczor et al Cancers (Basel). 2022 Jul 28;14(15). pii: cancers14153676. doi: 10.3390/cancers14153676.

Backbone

• Vector backbone: Lentivirus
• Backbone manufacturer: VectorBuilder
• Total vector size (bp): 9917
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: LivePAR(R163A)
• Alt name: WWE Domain
• Species: H. sapiens (human)
• Insert Size (bp): 252
• Mutation: R163A mutation in the WWE Domain
• Promoter: EF1A
• Tag / Fusion Protein:
  • EGFP (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Resource Information

• Supplemental Documents:
  • pLV-EF1A-LivePAR(R163A)-Hygro.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry