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Addgene

pEX128·gRNA
(Plasmid #187600)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187600 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEX-A128
  • Backbone manufacturer
    Eurofins

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    gRNA template
  • Alt name
    gRNA-csy4
  • gRNA/shRNA sequence
    CGACGGCCAGAAGATCGGTT
  • Promoter no promoter

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer ggagcagacaagcccgtcagg
  • 3′ sequencing primer caggctttacactttatgcttccggct
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Synthesised from Eurofins

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEX128·gRNA was a gift from Pablo Ivan Nikel (Addgene plasmid # 187600 ; http://n2t.net/addgene:187600 ; RRID:Addgene_187600)

  • For your References section:

    Modular (de)construction of complex bacterial phenotypes by CRISPR/nCas9-assisted, multiplex cytidine base-editing. Volke DC, Martino RA, Kozaeva E, Smania AM, Nikel PI. Nat Commun. 2022 May 31;13(1):3026. doi: 10.1038/s41467-022-30780-z. 10.1038/s41467-022-30780-z PubMed 35641501
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