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pcDNA5-FRT/TO-3*Flag-APEX2 C-term EWSR1
(Plasmid #187586)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187586 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA5/FRT/TO
  • Backbone manufacturer
    Thermofisher
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EWSR1
  • Species
    H. sapiens (human)
  • Entrez Gene
    EWSR1 (a.k.a. EWS, EWS-FLI1, bK984G1.4)
  • Promoter CMV
  • Tag / Fusion Protein
    • APEX2-FLAG (C terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The cDNA Gateway entry clone for this plasmid was provided by Dr. Anne-Claude Gingras' laboratory at Lunenfeld-Tanenbaum Research Institute.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5-FRT/TO-3*Flag-APEX2 C-term EWSR1 was a gift from Benjamin Blencowe (Addgene plasmid # 187586 ; http://n2t.net/addgene:187586 ; RRID:Addgene_187586)

  • For your References section:

    Systematic mapping of nuclear domain-associated transcripts reveals speckles and lamina as hubs of functionally distinct retained introns. Barutcu AR, Wu M, Braunschweig U, Dyakov BJA, Luo Z, Turner KM, Durbic T, Lin ZY, Weatheritt RJ, Maass PG, Gingras AC, Blencowe BJ. Mol Cell. 2022 Mar 3;82(5):1035-1052.e9. doi: 10.1016/j.molcel.2021.12.010. Epub 2022 Feb 18. 10.1016/j.molcel.2021.12.010 PubMed 35182477
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