pEPKKα1RKN0115
(Plasmid
#187571)
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PurposeTranscriptional unit for constitutive expression of a TALE driven by the nos promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 187571 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDGB3_alpha1R
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Vector typePlant Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTALE
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SpeciesSynthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BsaI (destroyed during cloning)
- 3′ cloning site BsaI (destroyed during cloning)
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2022.06.15.496242 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEPKKα1RKN0115 was a gift from Nicola Patron (Addgene plasmid # 187571 ; http://n2t.net/addgene:187571 ; RRID:Addgene_187571) -
For your References section:
Tunable control of insect pheromone biosynthesis in Nicotiana benthamiana. Kallam K, Moreno-Gimenez E, Mateos-Fernandez R, Tansley C, Gianoglio S, Orzaez D, Patron N. Plant Biotechnol J. 2023 Apr 9. doi: 10.1111/pbi.14048. 10.1111/pbi.14048 PubMed 37032497