pBabe p16 INK4A
(Plasmid
#18757)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 18757 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBabe puro
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Backbone manufacturerAvailable at Addgene (#1764)
- Backbone size w/o insert (bp) 5100
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Vector typeMammalian Expression, Retroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namep16 INK4a
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SpeciesH. sapiens (human)
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Entrez GeneCDKN2A (a.k.a. ARF, CAI2, CDK4I, CDKN2, CMM2, INK4, INK4A, MLM, MTS-1, MTS1, P14, P14ARF, P16, P16-INK4A, P16INK4, P16INK4A, P19, P19ARF, TP16)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer pBABE 5' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBabe p16 INK4A was a gift from Joan Brugge (Addgene plasmid # 18757) -
For your References section:
G1/S cell cycle arrest provides anoikis resistance through Erk-mediated Bim suppression. Collins NL, Reginato MJ, Paulus JK, Sgroi DC, Labaer J, Brugge JS. Mol Cell Biol. 2005 Jun . 25(12):5282-91. 10.1128/MCB.25.12.5282-5291.2005 PubMed 15923641