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Addgene

pEPCTαKN0001
(Plasmid #187568)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187568 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pDGB3_alpha1
  • Vector type
    Plant Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Luciferase
  • Species
    Synthetic

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (destroyed during cloning)
  • 3′ cloning site BsaI (destroyed during cloning)
  • 5′ sequencing primer Unknown
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Backbone contains a 1338 bp IS4 transposon, but its location is not an issue for plasmid functionality.

Please visit https://www.biorxiv.org/content/10.1101/2022.06.15.496242 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEPCTαKN0001 was a gift from Nicola Patron (Addgene plasmid # 187568 ; http://n2t.net/addgene:187568 ; RRID:Addgene_187568)
  • For your References section:

    Tunable control of insect pheromone biosynthesis in Nicotiana benthamiana. Kallam K, Moreno-Gimenez E, Mateos-Fernandez R, Tansley C, Gianoglio S, Orzaez D, Patron N. Plant Biotechnol J. 2023 Apr 9. doi: 10.1111/pbi.14048. 10.1111/pbi.14048 PubMed 37032497