pEPCTΩSP0022
(Plasmid
#187562)
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PurposeModule for copper-inducible expression of nanoluc luciferase and firefly luciferase driven by minimal synthetic promoters with binding sites for CUP2
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 187562 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDGB3_omega1
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Vector typePlant Expression
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFirefly Luciferase and NanoLuciferase
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SpeciesSynthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BsmBI (destroyed during cloning)
- 3′ cloning site BsmBI (destroyed during cloning)
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2022.06.15.496242 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEPCTΩSP0022 was a gift from Nicola Patron (Addgene plasmid # 187562 ; http://n2t.net/addgene:187562 ; RRID:Addgene_187562) -
For your References section:
Tunable control of insect pheromone biosynthesis in Nicotiana benthamiana. Kallam K, Moreno-Gimenez E, Mateos-Fernandez R, Tansley C, Gianoglio S, Orzaez D, Patron N. Plant Biotechnol J. 2023 Apr 9. doi: 10.1111/pbi.14048. 10.1111/pbi.14048 PubMed 37032497