1352wUV2 F3bbs kan NNNTGGGCGk
(Plasmid #18756)

• Depositing Lab: Scot Wolfe
• Publication: Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701.

Backbone

• Vector backbone: ColE1
• Backbone size w/o insert (bp): 3890
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Kanamycin, 100 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 3 tandem zinc fingers
• Species: M. musculus (mouse)
• Insert Size (bp): 1298
• Mutation: A Kan cassette (1090bp) is inserted between two bbsI sites in finger 3 of this construct. This cassette is here to provide a large drop out band when digested with BbsI. The digested backbone can then be used for building finger 3 libraries. Fingers one and two are anchor fingers which specify TGGGCGk.

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BbsI (not destroyed)
• 3′ cloning site: BbsI (not destroyed)
• 5′ sequencing primer: OMG5

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry