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1352wUV2 F1bbs kan GCGTGGNNN
(Plasmid #18753)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 18753 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    ColE1
  • Backbone size w/o insert (bp) 3890
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin, 100 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    3 tandem zinc fingers
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1298
  • Mutation
    A Kan cassette (1090bp) is inserted between two bbsI sites in finger 1 of this construct. This cassette is here to provide a large drop out band when digested with BbsI. The digested backbone can then be used for building finger 1 libraries. Fingers two and three are anchor fingers which specify GCGTGG.

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BbsI (not destroyed)
  • 3′ cloning site BbsI (not destroyed)
  • 5′ sequencing primer OMG5
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    1352wUV2 F1bbs kan GCGTGGNNN was a gift from Scot Wolfe (Addgene plasmid # 18753 ; http://n2t.net/addgene:18753 ; RRID:Addgene_18753)

  • For your References section:

    Targeted gene inactivation in zebrafish using engineered zinc-finger nucleases. Meng X, Noyes MB, Zhu LJ, Lawson ND, Wolfe SA. Nat Biotechnol. 2008 Jun . 26(6):695-701. 10.1038/nbt1398 PubMed 18500337
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