pWZL hygro H-Ras V12
(Plasmid #18749)

• Depositing Lab: Scott Lowe
• Publication: Serrano et al Cell. 1997 Mar 7. 88(5):593-602.

Backbone

• Vector backbone: pWZL Hygro
• Backbone manufacturer: Available at Addgene (#18750)
• Backbone size w/o insert (bp): 5915
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: H-Ras V12
• Alt name: HRas V12
• Species: H. sapiens (human)
• Insert Size (bp): 526
• Mutation: V12
• GenBank ID: NM_005343
• Entrez Gene: HRAS (a.k.a. C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: pBABE 5'

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Jay Morgenstern
• Articles Citing this Plasmid:
  • 16 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry