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Addgene

pEGPN-ACE2
(Plasmid #187483)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187483 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGPN
  • Backbone size w/o insert (bp) 7456
  • Total vector size (bp) 9915
  • Vector type
    Lentiviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ACE2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2448
  • Entrez Gene
    ACE2 (a.k.a. ACEH)
  • Promoter EFS

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CTAACTCGAACGCTAGCTGTGCGATCGTTT
  • 3′ sequencing primer AGGCCTCGGGATTCCTAGGAACAGCGGTTT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGPN-ACE2 was a gift from Lewis Cantley (Addgene plasmid # 187483 ; http://n2t.net/addgene:187483 ; RRID:Addgene_187483)

  • For your References section:

    Coagulation factors directly cleave SARS-CoV-2 spike and enhance viral entry. Kastenhuber ER, Mercadante M, Nilsson-Payant B, Johnson JL, Jaimes JA, Muecksch F, Weisblum Y, Bram Y, Chandar V, Whittaker GR, tenOever BR, Schwartz RE, Cantley L. Elife. 2022 Mar 23;11. pii: 77444. doi: 10.7554/eLife.77444. 10.7554/eLife.77444 PubMed 35294338
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