Skip to main content
Addgene

AAV.hSyn.GLPLight-ctr
(Plasmid #187468)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187468 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pAAV.hSynapsin1
  • Backbone manufacturer
    Viral Vector Facility - University of Zurich
  • Backbone size w/o insert (bp) 4443
  • Total vector size (bp) 6600
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GLPLight-ctr
  • Species
    Synthetic
  • Insert Size (bp)
    2157
  • GenBank ID
  • Promoter human Synapsin-1
  • Tag / Fusion Protein
    • Flag tag (N terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer ggagtcgtgtcgtgcctgag
  • 3′ sequencing primer aagcaatagcatgatacaaaggca
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.02.14.528498 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV.hSyn.GLPLight-ctr was a gift from Tommaso Patriarchi (Addgene plasmid # 187468 ; http://n2t.net/addgene:187468 ; RRID:Addgene_187468)

  • For your References section:

    Optical tools for visualizing and controlling human GLP-1 receptor activation with high spatiotemporal resolution. Duffet L, Williams ET, Gresch A, Chen S, Bhat MA, Benke D, Hartrampf N, Patriarchi T. Elife. 2023 Jun 2;12:RP86628. doi: 10.7554/eLife.86628. 10.7554/eLife.86628 PubMed 37265064
Related items:
Commonly requested with: