pBabe H-Ras V12 S35
(Plasmid
#18746)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 18746 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBabe-puro
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Backbone manufacturerAvailable at Addgene (#1764)
- Backbone size w/o insert (bp) 5100
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Vector typeMammalian Expression, Retroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameH-Ras V12 S35
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Alt nameHRas V12 S35
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Alt nameH-Ras
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SpeciesH. sapiens (human)
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Insert Size (bp)700
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MutationV12 S35
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GenBank IDNM_005343
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Entrez GeneHRAS (a.k.a. C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI / SalI (destroyed during cloning)
- 3′ cloning site BglII / BamHI (destroyed during cloning)
- 5′ sequencing primer pBabe 5'
- 3′ sequencing primer pBabe 3' (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byJay Morgenstern
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBabe H-Ras V12 S35 was a gift from Scott Lowe (Addgene plasmid # 18746 ; http://n2t.net/addgene:18746 ; RRID:Addgene_18746)
Map uploaded by the depositor.
![](https://media.addgene.org/data/easy-thumbnails/data/62/59/4e70055c-af62-11e0-90fe-003048dd6500.jpeg.940x940_q85_autocrop.png)