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Addgene

pBabe H-Ras V12 G37
(Plasmid #18745)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 18745 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBabe-puro
  • Backbone manufacturer
    Available at Addgene (#1764)
  • Backbone size w/o insert (bp) 5100
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    H-Ras V12 C40
  • Alt name
    HRas V12 C40
  • Alt name
    H-Ras
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    700
  • Mutation
    V12 / G37
  • GenBank ID
    NM_005343
  • Entrez Gene
    HRAS (a.k.a. C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI / SalI (destroyed during cloning)
  • 3′ cloning site BglII / BamHI (destroyed during cloning)
  • 5′ sequencing primer pBabe 5'
  • 3′ sequencing primer pBabe 3'
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Jay Morgenstern
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabe H-Ras V12 G37 was a gift from Scott Lowe (Addgene plasmid # 18745 ; http://n2t.net/addgene:18745 ; RRID:Addgene_18745)
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