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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 18744 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBabe-puro
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Backbone manufacturerAvailable at Addgene (#1764)
- Backbone size w/o insert (bp) 5100
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Vector typeMammalian Expression, Retroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameH-Ras
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Alt nameHRas
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SpeciesH. sapiens (human)
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Insert Size (bp)700
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MutationG12V
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GenBank IDNM_005343
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Entrez GeneHRAS (a.k.a. C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII / SnaBI (destroyed during cloning)
- 3′ cloning site HindIII / SnaBI (destroyed during cloning)
- 5′ sequencing primer pBabe 5'
- 3′ sequencing primer pBabe 3' (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byJay Morgenstern
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBabe H-Ras was a gift from Scott Lowe (Addgene plasmid # 18744 ; http://n2t.net/addgene:18744 ; RRID:Addgene_18744)
Map uploaded by the depositor.
