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Addgene

KEE +E111H
(Plasmid #187431)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187431 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFastBac Dual
  • Backbone manufacturer
    Thermo Fisher (Invitrogen)
  • Backbone size w/o insert (bp) 5238
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    ATP1A1
  • Species
    Rhabdophis subminiatus
  • Insert Size (bp)
    3069
  • Mutation
    changed glutamic acid at 117 to histidine
  • GenBank ID
    MT928191
  • Promoter PH

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ATTCCGGATTATTCATACCGTCCCACCATCG
  • 3′ sequencing primer GTGGTATGGCTGATTATGATCCTCTAGTACTTCTC
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    ATP1B1
  • Species
    Rhabdophis subminiatus
  • Insert Size (bp)
    915
  • GenBank ID
    ON168934
  • Promoter p10

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (unknown if destroyed)
  • 3′ cloning site PaeI (unknown if destroyed)
  • 5′ sequencing primer CGGGTTCCTTCCGGTATTGTCTCCTTC
  • 3′ sequencing primer ACGGACCTTTAATTCAACCCAACACAATATA
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    KEE +E111H was a gift from Susanne Dobler (Addgene plasmid # 187431 ; http://n2t.net/addgene:187431 ; RRID:Addgene_187431)

  • For your References section:

    Constraints on the evolution of toxin-resistant Na,K-ATPases have limited dependence on sequence divergence. Mohammadi S, Herrera-Álvarez S, Yang L, del Pilar Rodríguez-Ordoñez M, Zhang K, Storz JF, Dobler S, Crawford AJ, Andolfatto P. bioRxiv 2021.11.29.470343 10.1101/2021.11.29.470343
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