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pLPC H-Ras V12
(Plasmid #18741)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 18741 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pLPC
  • Backbone manufacturer
    Jay Morgenstern
  • Backbone size w/o insert (bp) 5763
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    H-Ras V12
  • Alt name
    HRas V12
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    526
  • Mutation
    V12
  • GenBank ID
    NM_005343
  • Entrez Gene
    HRAS (a.k.a. C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Jay Morgenstern
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLPC H-Ras V12 was a gift from Scott Lowe (Addgene plasmid # 18741 ; http://n2t.net/addgene:18741 ; RRID:Addgene_18741)
  • For your References section:

    Oncogenic ras provokes premature cell senescence associated with accumulation of p53 and p16INK4a. Serrano M, Lin AW, McCurrach ME, Beach D, Lowe SW. Cell. 1997 Mar 7. 88(5):593-602. 10.1016/S0092-8674(00)81902-9 PubMed 9054499