pLPC H-Ras V12
(Plasmid
#18741)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 18741 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLPC
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Backbone manufacturerJay Morgenstern
- Backbone size w/o insert (bp) 5763
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Vector typeMammalian Expression, Retroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameH-Ras V12
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Alt nameHRas V12
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SpeciesH. sapiens (human)
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Insert Size (bp)526
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MutationV12
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GenBank IDNM_005343
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Entrez GeneHRAS (a.k.a. C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byJay Morgenstern
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLPC H-Ras V12 was a gift from Scott Lowe (Addgene plasmid # 18741 ; http://n2t.net/addgene:18741 ; RRID:Addgene_18741) -
For your References section:
Oncogenic ras provokes premature cell senescence associated with accumulation of p53 and p16INK4a. Serrano M, Lin AW, McCurrach ME, Beach D, Lowe SW. Cell. 1997 Mar 7. 88(5):593-602. 10.1016/S0092-8674(00)81902-9 PubMed 9054499