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Addgene

pLPC 12S E1A
(Plasmid #18740)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 18740 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLPC
  • Backbone manufacturer
    Jay Morgenstern
  • Backbone size w/o insert (bp) 5763
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    12S E1A
  • Alt name
    E1A 12S
  • Species
    adenovirus
  • Insert Size (bp)
    973
  • GenBank ID
    AAQ72378

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Jay Morgenstern

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLPC 12S E1A was a gift from Scott Lowe (Addgene plasmid # 18740 ; http://n2t.net/addgene:18740 ; RRID:Addgene_18740)

  • For your References section:

    Selective induction of p53 and chemosensitivity in RB-deficient cells by E1A mutants unable to bind the RB-related proteins. Samuelson AV, Lowe SW. Proc Natl Acad Sci U S A. 1997 Oct 28. 94(22):12094-9. 10.1073/pnas.94.22.12094 PubMed 9342368
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