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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 18740 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLPC
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Backbone manufacturerJay Morgenstern
- Backbone size w/o insert (bp) 5763
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Vector typeMammalian Expression, Retroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name12S E1A
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Alt nameE1A 12S
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Speciesadenovirus
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Insert Size (bp)973
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GenBank IDAAQ72378
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byJay Morgenstern
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLPC 12S E1A was a gift from Scott Lowe (Addgene plasmid # 18740 ; http://n2t.net/addgene:18740 ; RRID:Addgene_18740) -
For your References section:
Selective induction of p53 and chemosensitivity in RB-deficient cells by E1A mutants unable to bind the RB-related proteins. Samuelson AV, Lowe SW. Proc Natl Acad Sci U S A. 1997 Oct 28. 94(22):12094-9. 10.1073/pnas.94.22.12094 PubMed 9342368
Map uploaded by the depositor.
![](https://media.addgene.org/data/easy-thumbnails/data/22/79/4e32366e-af62-11e0-90fe-003048dd6500.jpeg.940x940_q85_autocrop.png)