R6K_BAD_GFP-mCherry_ChInt++
(Plasmid #187393)

• Purpose: chromosomal integration downstream of glmS gene via Tn7 system of a cassette encoding for arabinose-inducible GFP and mCherry
• Depositing Lab: Roi Avraham
• Publication: Bacterial fluorescent reporters and tags toolkit (unpublished)

Backbone

• Vector backbone: R6Kgamma
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Amp + Kan + Dap
• Growth Temperature: 37°C
• Growth Strain(s): MFDpir
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: GFP, mCherry
• Species: Synthetic

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Supplemental Documents:
  • R6K_BAD_GFP-mCherry_ChInt++.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid requires a pir+ strain for growth and transformation. It will be distributed in MFDpir E. coli. MFDpir strain is auxotrophic for DAP (diaminopimelic acid), so it must be added to the media to grow and extract the plasmid (0.3 mM working concentration).