Skip to main content
Addgene

R6K_tac_dGFPmut3_ChInt
(Plasmid #187388)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187388 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    R6Kgamma
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Amp + Kan + Dap
  • Growth Temperature
    37°C
  • Growth Strain(s)
    MFDpir
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    dGFPmut3_AAV
  • Species
    Synthetic
  • Mutation
    C-terminal AAV tail
  • Tag / Fusion Protein
    • AAV (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid requires a pir+ strain for growth and transformation. It will be distributed in MFDpir E. coli. MFDpir strain is auxotrophic for DAP (diaminopimelic acid), so it must be added to the media to grow and extract the plasmid (0.3 mM working concentration).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    R6K_tac_dGFPmut3_ChInt was a gift from Roi Avraham (Addgene plasmid # 187388 ; http://n2t.net/addgene:187388 ; RRID:Addgene_187388)
Related items: