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Addgene

pKD4_mCherry
(Plasmid #187386)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187386 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pkD4
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Amp + Kan + Dap
  • Growth Temperature
    37°C
  • Growth Strain(s)
    MFDpir
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    mCherry
  • Species
    Synthetic

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid requires a pir+ strain for growth and transformation. It will be distributed in MFDpir E. coli. MFDpir strain is auxotrophic for DAP (diaminopimelic acid), so it must be added to the media to grow and extract the plasmid (0.3 mM working concentration).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKD4_mCherry was a gift from Roi Avraham (Addgene plasmid # 187386 ; http://n2t.net/addgene:187386 ; RRID:Addgene_187386)
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