pKD3_mCherry
(Plasmid
#187385)
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PurposeTo chromosomally tag genes of interest with mCherry. FRT-mCherry-cat-FRT
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 187385 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepKD3
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Amp + Chl + Dap
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Growth Temperature37°C
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Growth Strain(s)MFDpir
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namemCherry
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SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid requires a pir+ strain for growth and transformation. It will be distributed in MFDpir E. coli. MFDpir strain is auxotrophic for DAP (diaminopimelic acid), so it must be added to the media to grow and extract the plasmid (0.3 mM working concentration).
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKD3_mCherry was a gift from Roi Avraham (Addgene plasmid # 187385 ; http://n2t.net/addgene:187385 ; RRID:Addgene_187385)
