pFPV25.1_dGFPmut3-LVA
(Plasmid
#187378)
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PurposerpsM promoter driving the expression of destabilized GFPmut3 (containing a LVA C-terminal tail)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 187378 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepFPV25.1
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namedGFPmut3_LVA
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SpeciesSynthetic
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MutationC-terminal LVA tail
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Tag
/ Fusion Protein
- LVA (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFPV25.1_dGFPmut3-LVA was a gift from Roi Avraham (Addgene plasmid # 187378 ; http://n2t.net/addgene:187378 ; RRID:Addgene_187378)