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Cherry-Plekhh1 5 silent mutations
(Plasmid #187373)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187373 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmCherry-C1
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Plekhh1 with 5 silent mutations
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4095
  • Mutation
    Five silent mutations at the Plekhh1 siRNA site (CAGGAAGACCAGCGGACTA) to CAG[A]AA[A]AC[A]AG[T]GG[T]CTA
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Cherry-Plekhh1 5 silent mutations was a gift from Evelyne Coudrier (Addgene plasmid # 187373 ; http://n2t.net/addgene:187373 ; RRID:Addgene_187373)

  • For your References section:

    Plekhh1, a partner of myosin 1 and an effector of EphB2, controls the cortical actin network during cell repulsion. Prosperi MT, Pernier J, Lachuer H, Coudrier E. J Cell Sci. 2021 Dec 1;134(23). pii: 272686. doi: 10.1242/jcs.258802. Epub 2021 Dec 6. 10.1242/jcs.258802 PubMed 34723325
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