EGFP-Plekhh1-NTer
(Plasmid
#187368)
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PurposeExpresses human Plekhh1-NTer labelled with EGFP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 187368 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4728
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePlekhh1-NTer
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SpeciesH. sapiens (human)
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Insert Size (bp)1620
- Promoter CMV
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Tag
/ Fusion Protein
- EGFP (N terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer EGFP-N (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EGFP-Plekhh1-NTer was a gift from Evelyne Coudrier (Addgene plasmid # 187368 ; http://n2t.net/addgene:187368 ; RRID:Addgene_187368) -
For your References section:
Plekhh1, a partner of myosin 1 and an effector of EphB2, controls the cortical actin network during cell repulsion. Prosperi MT, Pernier J, Lachuer H, Coudrier E. J Cell Sci. 2021 Dec 1;134(23). pii: 272686. doi: 10.1242/jcs.258802. Epub 2021 Dec 6. 10.1242/jcs.258802 PubMed 34723325