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Addgene

EGFP-Myo1b
(Plasmid #187362)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187362 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4752
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    myosin 1b isoform b
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    3324
  • Entrez Gene
    Myo1b (a.k.a. Myr1)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer EGFP-N
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The expression of the recombinant plasmid includes the addition of 18 amino acids (SGLRSRAQASNSDPRYPP) between GFP and Myo1b.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EGFP-Myo1b was a gift from Evelyne Coudrier (Addgene plasmid # 187362 ; http://n2t.net/addgene:187362 ; RRID:Addgene_187362)

  • For your References section:

    Association of myosin I alpha with endosomes and lysosomes in mammalian cells. Raposo G, Cordonnier MN, Tenza D, Menichi B, Dürrbach A, Louvard D, Coudrier E. Mol Biol Cell. 1999 May . 10(5):1477-94. PubMed 10233157
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