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Addgene

pTripz Ctrl-IRES-mCherry
(Plasmid #187295)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187295 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTripz
  • Backbone size w/o insert (bp) 12718
  • Vector type
    Lentiviral
  • Selectable markers
    tetracycline

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IRES-mCherry
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    587
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Plasmid made by Alexis de Sa Ribeiro Do Bonfim Melo

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

5' cloning site: Age1 (not destroyed), 3' cloning site: Cla1 (not destroyed).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTripz Ctrl-IRES-mCherry was a gift from Alpha Yap (Addgene plasmid # 187295 ; http://n2t.net/addgene:187295 ; RRID:Addgene_187295)

  • For your References section:

    Rapid lamellipodial responses by neighbor cells drive epithelial sealing in response to pyroptotic cell death. Bonfim-Melo A, Noordstra I, Gupta S, Chan AH, Jones MJK, Schroder K, Yap AS. Cell Rep. 2022 Feb 1;38(5):110316. doi: 10.1016/j.celrep.2022.110316. 10.1016/j.celrep.2022.110316 PubMed 35108534
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