pTripz Casp1-IRES-mCherry
(Plasmid #187294)

• Purpose: This is Caspase1 from mouse (NM_009807.2) with its CARD domain replaced by the FKBP-V domain (dimerising domain).
• Depositing Lab: Alpha Yap
• Publication: Bonfim-Melo et al Cell Rep. 2022 Feb 1;38(5):110316. doi: 10.1016/j.celrep.2022.110316.

Backbone

• Vector backbone: pTripz
• Backbone size w/o insert (bp): 12677
• Vector type: Lentiviral
• Selectable markers: tetracycline

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Caspase1
• Species: M. musculus (mouse)
• Insert Size (bp): 1873
• Entrez Gene: Casp1 (a.k.a. ICE, Il1bc)
• Promoter: CMV
• Tag / Fusion Protein:
  • mCherry (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: mCherry-R

Resource Information

• Supplemental Documents:
  • pTripz Casp1-IRES-mCherry.gb
• A portion of this plasmid was derived from a plasmid made by: Plasmid made by Alexis de Sa Ribeiro Do Bonfim Melo

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

5' cloning site: Age1 (not destroyed), 3' cloning site: Cla1 (not destroyed).