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GLUT1-eGFP/pcDNA-DEST47
(Plasmid #18729)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 18729 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA-DEST47
  • Backbone manufacturer
    invitrogen
  • Backbone size w/o insert (bp) 7780
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pcDNA-DEST47 /hGLUT1-eGFP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1500
  • Mutation
    deleted TAA
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site Gateway (not destroyed)
  • 3′ cloning site Gateway (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GLUT1-eGFP/pcDNA-DEST47 was a gift from Wolf Frommer (Addgene plasmid # 18729 ; http://n2t.net/addgene:18729 ; RRID:Addgene_18729)

  • For your References section:

    GLUT1 and GLUT9 as major contributors to glucose influx in HepG2 cells identified by a high sensitivity intramolecular FRET glucose sensor. Takanaga H, Chaudhuri B, Frommer WB. Biochim Biophys Acta. 2008 Apr . 1778(4):1091-9. 10.1016/j.bbamem.2007.11.015 PubMed 18177733
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