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IL2-AH-2A-Cherry-RhoAQ63L in pmCherryN1
(Plasmid #187287)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187287 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmCherry-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4694
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    interleukin-2 receptor alpha-subunit, anillin, RhoA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2403
  • Mutation
    AH-2A-RhoA Q63L
  • Entrez Gene
    IL2 (a.k.a. IL-2, TCGF, lymphokine)
  • Entrez Gene
    ANLN (a.k.a. FSGS8, Scraps, scra)
  • Entrez Gene
    RHOA (a.k.a. ARH12, ARHA, EDFAOB, RHO12, RHOH12)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Plasmid made by Srikanth Budnar

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

5' cloning site: Nhe1 (destroyed), 3' cloning site: Not1 (not destroyed).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    IL2-AH-2A-Cherry-RhoAQ63L in pmCherryN1 was a gift from Alpha Yap (Addgene plasmid # 187287 ; http://n2t.net/addgene:187287 ; RRID:Addgene_187287)

  • For your References section:

    Anillin Promotes Cell Contractility by Cyclic Resetting of RhoA Residence Kinetics. Budnar S, Husain KB, Gomez GA, Naghibosadat M, Varma A, Verma S, Hamilton NA, Morris RG, Yap AS. Dev Cell. 2019 Jun 17;49(6):894-906.e12. doi: 10.1016/j.devcel.2019.04.031. Epub 2019 May 16. 10.1016/j.devcel.2019.04.031 PubMed 31105010
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