pEGFP-SRGAP1
(Plasmid #187269)

• Purpose: Expression of SRGAP1-EGFP
• Depositing Lab: Alpha Yap
• Publication: Liang et al Nat Commun. 2017 Oct 5;8(1):790. doi: 10.1038/s41467-017-00797-w.

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SRGAP1
• Alt name: SRGAP1 (a.k.a. ARHGAP13, FLJ22166, KIAA1304)
• Species: H. sapiens (human)
• Insert Size (bp): 3255
• Entrez Gene: SRGAP1 (a.k.a. ARHGAP13, FLJ22166, KIAA1304)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xho1 (not destroyed)
• 3′ cloning site: BamH1 (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: EGFP Rev Seq Primer

Resource Information

• Supplemental Documents:
  • pEGFP-SRGAP1.gb
• A portion of this plasmid was derived from a plasmid made by: Plasmid made by Xuan Liang

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry