Skip to main content
Addgene

pLL5.0 CortW22A mCherry
(Plasmid #187268)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187268 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pLL5.0
  • Backbone size w/o insert (bp) 7554
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cortactin
  • Alt name
    CTTN (a.k.a. EMS1)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1650
  • Mutation
    W22A
  • Entrez Gene
    CTTN (a.k.a. EMS1)
  • Promoter 5' LTR
  • Tag / Fusion Protein
    • mcherry (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site BamH1 (not destroyed)
  • 3′ sequencing primer mCherry-R
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Plasmid made by Siew Ping/Xuan Liang

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLL5.0 CortW22A mCherry was a gift from Alpha Yap (Addgene plasmid # 187268 ; http://n2t.net/addgene:187268 ; RRID:Addgene_187268)

  • For your References section:

    Tyrosine dephosphorylated cortactin downregulates contractility at the epithelial zonula adherens through SRGAP1. Liang X, Budnar S, Gupta S, Verma S, Han SP, Hill MM, Daly RJ, Parton RG, Hamilton NA, Gomez GA, Yap AS. Nat Commun. 2017 Oct 5;8(1):790. doi: 10.1038/s41467-017-00797-w. 10.1038/s41467-017-00797-w PubMed 28983097
Related items: