pLL5.0 hCortKD EGFP
(Plasmid #187267)

• Purpose: Lentiviral expression of shRNA targeting Cortactin
• Depositing Lab: Alpha Yap
• Publication: Liang et al Nat Commun. 2017 Oct 5;8(1):790. doi: 10.1038/s41467-017-00797-w.

Backbone

• Vector backbone: pLL5.0
• Backbone size w/o insert (bp): 7570
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: shRNA for Cortactin
• gRNA/shRNA sequence: GAGAAGCACGAGTCACAGA
• Species: H. sapiens (human)
• Insert Size (bp): 53
• Entrez Gene: CTTN (a.k.a. EMS1)
• Promoter: U6
• Tag / Fusion Protein:
  • EGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Hpa1 (destroyed during cloning)
• 3′ cloning site: Xho1 (not destroyed)
• 5′ sequencing primer: mU6 Forward Primer

Resource Information

• Supplemental Documents:
  • pLL5.0 hCortKD EGFP.gb
• A portion of this plasmid was derived from a plasmid made by: Plasmid made by Siew Ping/Xuan Liang

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry