pLL5.0 hCortKD-Flcortmut3YF mCherry
(Plasmid #187265)

• Purpose: Lentiviral expression of shRNA targeting Cortactin + reconstitution of Cortactin3YF mutant
• Depositing Lab: Alpha Yap
• Publication: Liang et al Nat Commun. 2017 Oct 5;8(1):790. doi: 10.1038/s41467-017-00797-w.

Backbone

• Vector backbone: pLL5.0
• Backbone size w/o insert (bp): 7550
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cortactin
• Alt name: CTTN (a.k.a. EMS1)
• gRNA/shRNA sequence: GAGAAGCACGAGTCACAGA
• Species: H. sapiens (human)
• Insert Size (bp): 1703
• Mutation: 3YF (Y412, Y470, Y486)
• Entrez Gene: CTTN (a.k.a. EMS1)
• Promoter: U6, 5'LTR
• Tag / Fusion Protein:
  • mCherry (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1 (not destroyed)
• 3′ cloning site: BamH1 (not destroyed)
• 5′ sequencing primer: mU6 Forward Primer
• 3′ sequencing primer: mCherry-R

Resource Information

• Supplemental Documents:
  • pLL5.0 hCortKD-Flcortmut3YF mCherry.gb
• A portion of this plasmid was derived from a plasmid made by: Plasmid made by Siew Ping/Xuan Liang

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Rescue with reconstituted Cortactin 3YF mutant