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pLL5.0-MRLC-AA-GFP
(Plasmid #187259)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187259 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLL5.0
  • Backbone size w/o insert (bp) 6850
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MRLC1
  • Alt name
    MYL9 (a.k.a. LC20, MLC-2C, MLC2, MMIHS4, MRLC1, MYRL2)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1249
  • Mutation
    T18A,S19A
  • Entrez Gene
    MYL9 (a.k.a. LC20, MLC-2C, MLC2, MMIHS4, MRLC1, MYRL2)
  • Promoter 5' LTR
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site BsrG1 (not destroyed)
  • 5′ sequencing primer MRLC-AA Fwd Seq Primer
  • 3′ sequencing primer EGFP Rev Seq Primer
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Plasmid made by Kenneth Wee

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLL5.0-MRLC-AA-GFP was a gift from Alpha Yap (Addgene plasmid # 187259 ; http://n2t.net/addgene:187259 ; RRID:Addgene_187259)

  • For your References section:

    Snail induces epithelial cell extrusion by regulating RhoA contractile signalling and cell-matrix adhesion. Wee K, Hediyeh-Zadeh S, Duszyc K, Verma S, N Nanavati B, Khare S, Varma A, Daly RJ, Yap AS, Davis MJ, Budnar S. J Cell Sci. 2020 Jul 6;133(13). pii: jcs.235622. doi: 10.1242/jcs.235622. 10.1242/jcs.235622 PubMed 32467325
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