pLL5.0 shCAV1-NLS-mCherry
(Plasmid #187257)

• Purpose: Lentiviral expression of shRNA targeting Caveolin1 and NLS-mCherry
• Depositing Lab: Alpha Yap
• Publication: Katsuno-Kambe et al Biol Cell. 2021 Feb;113(2):107-117. doi: 10.1111/boc.202000116. Epub 2020 Nov 25.

Backbone

• Vector backbone: pLL5.0
• Backbone size w/o insert (bp): 7917
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ShRNA for Caveolin-1
• gRNA/shRNA sequence: GCATCTTTCTCTCTGTTAA
• Species: M. musculus (mouse)
• Insert Size (bp): 59
• Entrez Gene: Cav1 (a.k.a. Cav, Cav-1)
• Promoter: U6
• Tag / Fusion Protein:
  • NLS-mCherry (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Hpa1 (destroyed during cloning)
• 3′ cloning site: Xho1 (not destroyed)
• 5′ sequencing primer: mU6 Forward Primer
• 3′ sequencing primer: mCherry-R

Resource Information

• Supplemental Documents:
  • pLL5.0 shCAV1-NLS-mCherry.gb
• A portion of this plasmid was derived from a plasmid made by: Plasmid made by Jessica Teo

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry