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pLL5.0 shFmnl2-EGFP
(Plasmid #187255)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187255 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLL5.0
  • Backbone size w/o insert (bp) 7565
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ShRNA for Fmnl2
  • Alt name
    formin like 2
  • gRNA/shRNA sequence
    TGAGAGGTCTTCTAAGAAA
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    58
  • Entrez Gene
    Fmnl2 (a.k.a. 5430425K04Rik, Man)
  • Promoter U6
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Hpa1 (destroyed during cloning)
  • 3′ cloning site Xho1 (not destroyed)
  • 5′ sequencing primer mU6 Forward Primer
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Plasmid made by Jessica Teo

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLL5.0 shFmnl2-EGFP was a gift from Alpha Yap (Addgene plasmid # 187255 ; http://n2t.net/addgene:187255 ; RRID:Addgene_187255)

  • For your References section:

    Caveolin-1 influences epithelial collective cell migration via FMNL2 formin. Katsuno-Kambe H, Parton RG, Yap AS, Teo JL. Biol Cell. 2021 Feb;113(2):107-117. doi: 10.1111/boc.202000116. Epub 2020 Nov 25. 10.1111/boc.202000116 PubMed 33169848
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