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pLL5.0 ShCavin1-EGFP
(Plasmid #187253)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187253 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLL5.0
  • Backbone size w/o insert (bp) 7569
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ShRNA for Cavin1
  • gRNA/shRNA sequence
    TGAGCAGCCGGACCAATAA
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    54
  • Entrez Gene
    Cavin1 (a.k.a. 2310075E07Rik, Cav-p60, Cavin, Ptrf)
  • Promoter U6
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Hpa1 (destroyed during cloning)
  • 3′ cloning site Xho1 (not destroyed)
  • 5′ sequencing primer mU6 Forward Primer
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Plasmid made by Jessica Teo

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLL5.0 ShCavin1-EGFP was a gift from Alpha Yap (Addgene plasmid # 187253 ; http://n2t.net/addgene:187253 ; RRID:Addgene_187253)

  • For your References section:

    Caveolae Control Contractile Tension for Epithelia to Eliminate Tumor Cells. Teo JL, Gomez GA, Weeratunga S, Davies EM, Noordstra I, Budnar S, Katsuno-Kambe H, McGrath MJ, Verma S, Tomatis V, Acharya BR, Balasubramaniam L, Templin RM, McMahon KA, Lee YS, Ju RJ, Stebhens SJ, Ladoux B, Mitchell CA, Collins BM, Parton RG, Yap AS. Dev Cell. 2020 Jul 6;54(1):75-91.e7. doi: 10.1016/j.devcel.2020.05.002. Epub 2020 Jun 1. 10.1016/j.devcel.2020.05.002 PubMed 32485139
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