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Dual sgRNA CRISPRa Libraries
(Pooled Library #187249, #187250, #187251, #1000000193)

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  • Purpose

    Ultra-compact, human genome-wide CRISPRa library targeting each gene with a single element encoding a dual sgRNA cassette.

  • Vector Backbone

    pJR104 (Plasmid #187243) - does not express Cas9

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 1000000193 Three tubes, each with a third of the library (Library 1-2, 3-4, and 5-6) in backbone pJR104
  • #187249 (Library 1-2)
  • #187250 (Library 3-4)
  • #187251 (Library 5-6)
 1 $1300 Add to Cart
Pooled Library 187249 hCRISPRa_dual_1_2 pooled library† 1 $540 Add to Cart
Pooled Library 187250 hCRISPRa_dual_3_4 pooled library† 1 $540 Add to Cart
Pooled Library 187251 hCRISPRa_dual_5_6 pooled library† 1 $540 Add to Cart
Available to Academic and Nonprofits Only

  • † A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used in conjunction with cell lines already expressing an activator Cas9.

Library Details

  • Genes/gRNAs/plasmids
    21,503 dual sgRNA elements
  • Controls
    1,023 dual sgRNA control elements
  • Lentiviral Generation
    3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

  • Volume
    ∼20 µL
  • Concentration
    50 ng/µL

Resource Information

Depositor Comments

User instructions are available on GitHub ( https://github.com/josephreplogle/CRISPRi-dual-sgRNA-screens).

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Dual sgRNA CRISPRa libraries were a gift from Jonathan Weissman (Addgene #1000000193)
    Dual sgRNA CRISPRa Library 1-2 was a gift from Jonathan Weissman (Addgene #187249)
    Dual sgRNA CRISPRa Library 3-4 was a gift from Jonathan Weissman (Addgene #187250)
    Dual sgRNA CRISPRa Library 5-6 was a gift from Jonathan Weissman (Addgene #187251)

  • For your References section:

    Maximizing CRISPRi efficacy and accessibility with dual-sgRNA libraries and optimal effectors. Replogle JM, Bonnar JL, Pogson AN, Liem CR, Maier NK, Ding Y, Russell BJ, Wang X, Leng K, Guna A, Norman TM, Pak RA, Ramos DM, Ward ME, Gilbert LA, Kampmann M, Weissman JS, Jost M. bioRxiv 2022.07.13.499814. doi: 10.1101/2022.07.13.499814. bioRxiv
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