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Addgene

pJR98
(Plasmid #187239)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187239 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUCIDT
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CR3 constant region - hU6 sgRNA promoter flanked by BsmBI sites
  • Species
    Synthetic

Cloning Information

  • Cloning method Unknown

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2022.07.13.499814 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJR98 was a gift from Marco Jost & Jonathan Weissman (Addgene plasmid # 187239 ; http://n2t.net/addgene:187239 ; RRID:Addgene_187239)

  • For your References section:

    Maximizing CRISPRi efficacy and accessibility with dual-sgRNA libraries and optimal effectors. Replogle JM, Bonnar JL, Pogson AN, Liem CR, Maier NK, Ding Y, Russell BJ, Wang X, Leng K, Guna A, Norman TM, Pak RA, Ramos DM, Ward ME, Gilbert LA, Kampmann M, Weissman JS, Jost M. eLife. 2022 Dec 28;11:e81856. doi: 10.7554/eLife.81856. 10.7554/eLife.81856 PubMed 36576240
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